• Featured Product
  • KD/KO Validated

RHEB Polyclonal antibody

RHEB Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15924-1-AP

Print datasheet

Synonyms

RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, GTP binding protein Rheb, EC:3.6.5.-



经过测试的应用

Positive WB detected inDU 145 cells, human brain tissue, HeLa cells, 3T3-L1 cells, fetal human brain tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pancreas cancer tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RHEB Ready-To-Use IHC Kit. RHEB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15924-1-AP targets RHEB in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen RHEB fusion protein Ag8747 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ras homolog enriched in brain
Synonyms RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, GTP binding protein Rheb, EC:3.6.5.-
Calculated Molecular Weight 184 aa, 20 kDa
Observed Molecular Weight 15-20 kDa
GenBank Accession NumberBC016155
Gene Symbol RHEB
Gene ID (NCBI) 6009
RRIDAB_2285084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15382
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.

实验方案

Product Specific Protocols
WB protocol for RHEB antibody 15924-1-APDownload protocol
IHC protocol for RHEB antibody 15924-1-APDownload protocol
IF protocol for RHEB antibody 15924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Cell Metab

Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.

Authors - Noriaki Shimizu
mouseIHC

PLoS Biol

RHEB/mTOR hyperactivity causes cortical malformations and epileptic seizures through increased axonal connectivity.

Authors - Martina Proietti Onori
mouseIF

Metabolism

Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression.

Authors - Yan Yang
  • KO Validated
mouseIF

iScience

Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9

Authors - Ting Wang
human

Front Cell Dev Biol

PPT1 Reduction Contributes to Erianin-Induced Growth Inhibition in Oral Squamous Carcinoma Cells.

Authors - Qingqiong Luo
mouseWB

Stem Cell Res Ther

Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.

Authors - Caixia Jin