验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse placenta tissue, MCF-7 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TPT1 Ready-To-Use IHC Kit. TPT1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10824-1-AP targets TPT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | TPT1 fusion protein Ag1268 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor protein, translationally-controlled 1 |
| 别名 | Translationally-controlled tumor protein, TCTP, p23, HRF, Histamine-releasing factor |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22-25 kDa |
| GenBank蛋白编号 | BC003352 |
| 基因名称 | TPT1 |
| Gene ID (NCBI) | 7178 |
| RRID | AB_2287617 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13693 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22-25 kDa TPT1 in lysates. An additional band around 50 kDa can also be observed occasionally, possibly representing the dimer form of TPT1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TPT1 antibody 10824-1-AP | Download protocol |
| IHC protocol for TPT1 antibody 10824-1-AP | Download protocol |
| IF protocol for TPT1 antibody 10824-1-AP | Download protocol |
| IP protocol for TPT1 antibody 10824-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Pharmacol Res Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect. | ||
Int J Biol Sci Ailanthus Altissima-derived Ailanthone enhances Gastric Cancer Cell Apoptosis by Inducing the Repression of Base Excision Repair by Downregulating p23 Expression. | ||
Chem Biol Interact AMG900 as novel inhibitor of the translationally controlled tumor protein. | ||
Invest New Drugs A novel ligand of the translationally controlled tumor protein (TCTP) identified by virtual drug screening for cancer differentiation therapy. | ||
BMC Cancer Identification of proteins involved in neural progenitor cell targeting of gliomas. | ||
Cancer Sci LncRNA TPT1-AS1 promotes tumorigenesis and metastasis in epithelial ovarian cancer by inducing TPT1 expression.
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