验证数据展示
经过测试的应用
| Positive WB detected in | PC-12 cells, fetal human brain tissue, mouse brain tissue, pig brain tissue, rat brain tissue, SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy STMN2 Ready-To-Use IHC Kit. STMN2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
67204-1-Ig targets STMN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 | STMN2 fusion protein Ag28990 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | stathmin-like 2 |
| 别名 | SGC 10, SCGN10, SCGN 10, SCG10, SCG 10 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC006302 |
| 基因名称 | STMN2 |
| Gene ID (NCBI) | 11075 |
| RRID | AB_2882497 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q93045 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STMN2 gene encodes a SCG10 (superior cervical ganglion-10) protein, a regulator of microtubule stability. When phosphorylated by MAPK8, SCG10 stabilizes microtubules and consequently controls neurite length in cortical neurons. SCG10 was early considered a neuron-specific growth-associated protein in Alzheimer's disease. In the developing brain, SCG10 negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STMN2 antibody 67204-1-Ig | Download protocol |
| IHC protocol for STMN2 antibody 67204-1-Ig | Download protocol |
| IF protocol for STMN2 antibody 67204-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
