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TGF Beta 1 Polyclonal antibody

TGF Beta 1 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IF/ICC, IF-P, ELISA and More (5)

Conjugate

Unconjugated

Cat no : 21898-1-AP

Print datasheet

Synonyms

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, Tgfb 1, TGFB



经过测试的应用

Positive WB detected inmouse skin tissue, A549 cells, HeLa cells, K-562 cells, L-929 cells, mouse spleen tissue, rat skin tissue
Positive IF-P detected inhuman stomach cancer tissue
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21898-1-AP targets TGF Beta 1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ELISA, Cell treatment
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, zebrafish, duck
Immunogen TGF Beta 1 fusion protein Ag13591 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transforming growth factor, beta 1
Synonyms TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, Tgfb 1, TGFB
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC000125
Gene Symbol TGFB1
Gene ID (NCBI) 7040
RRIDAB_2811115
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01137
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody detects the pro-TGF beta 1 and the cleaved fragment Latency-associated peptide.

实验方案

Product Specific Protocols
WB protocol for TGF Beta 1 antibody 21898-1-APDownload protocol
IF protocol for TGF Beta 1 antibody 21898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Adv Mater

Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine

Authors - Peng Lou
humanWB

ACS Nano

Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.

Authors - Meng Zhao
humanWB

Blood

C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse

Authors - Li-Xue Yang
WB,IF

Acta Pharm Sin B

Fucoidan-functionalized activated platelet-hitchhiking micelles simultaneously track tumor cells and remodel the immunosuppressive microenvironment for efficient metastatic cancer treatment.

Authors - Rong Guo
humanWB

Dev Cell

Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation

Authors - Bin Tan
humanWB

Redox Biol

Lactate-induced activation of tumor-associated fibroblasts and IL-8-mediated macrophage recruitment promote lung cancer progression

Authors - Xuyu Gu