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TUBB3-specific/TUJ1 Monoclonal antibody

TUBB3-specific/TUJ1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1F8G10

Cat no : 66375-1-Ig

Print datasheet

Synonyms

TUBB3, TUJ1, beta tubulin, Tubulin beta III, Tubulin beta 3 chain

验证数据展示

  • IF Staining of Retinal organoids using 66375-1-Ig

    IF Staining of Retinal organoids using 66375-1-Ig

    Retinal organoids (day 60) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and Cytokeratin 19 using 10712-1-AP at 1:200 (red). Nuclear stain DAPI (blue). Scale bar = 100 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

  • IF Staining of iPS cells using 66375-1-Ig

    IF Staining of iPS cells using 66375-1-Ig

    Immunofluorescent staining of TUBB3 (66375-1-lg, 1:250) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (Green: TUBB3; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

  • IF Staining of human neuron using 66375-1-Ig

    IF Staining of human neuron using 66375-1-Ig

    Immunofluorescence analysis of human pluripotent stem cell-derived astrocytes with S100β (15146-1-AP) at 1/200 (Magenta) and neurons with TUJ1 (66375-1-Ig) at 1:500 (Green). The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.3% Triton X-100. Alexa Fluor 488-conjugated goat anti-mouse IgG (1/500) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1/500) were used as the secondary antibodies. Nuclei were counterstained with DAPI (blue).

  • IF Staining of Retinal organoids using 66375-1-Ig

    IF Staining of Retinal organoids using 66375-1-Ig

    Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and PAX6 (12323-1-AP) at 1:500. Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

  • IF Staining of rat brain using 66375-1-Ig

    IF Staining of rat brain using 66375-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66375-1-Ig (TUBB3-specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 26975-1-AP (NeuN antibody, red).

  • WB analysis using 66375-1-Ig

    WB analysis using 66375-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:49000 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 66375-1-Ig

    FC experiment of SH-SY5Y using 66375-1-Ig

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human TUBB3-specific (66375-1-Ig, Clone:1F8G10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of mouse brain using 66375-1-Ig

    IHC staining of mouse brain using 66375-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IHC staining of human cerebellum using 66375-1-Ig

    IHC staining of human cerebellum using 66375-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 10x lens).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IHC staining of human cerebellum using 66375-1-Ig

    IHC staining of human cerebellum using 66375-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 40x lens).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • WB analysis of fetal human brain using 66375-1-Ig

    WB analysis of fetal human brain using 66375-1-Ig

    Western blot analysis of TUBB3 in various tissues and cell lines with 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:40,000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 66375-1-Ig

    IHC staining of human cerebellum using 66375-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TUBB3 antibody included
  • IHC staining of human cerebellum using 66375-1-Ig

    IHC staining of human cerebellum using 66375-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TUBB3 antibody included

经过测试的应用

Positive WB detected inSH-SY5Y cells, PC-12 cells, Neuro-2a cells, Pig brain, Rabbit brain, Rat brain Mouse brain, Chicken brain, human brain tissue
Positive IHC detected inhuman cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue, Retinal organoids, mouse brain tissue, human neuron
Positive IF/ICC detected iniPS cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:20000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66375-1-Ig targets TUBB3-specific/TUJ1 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Cited Reactivityhuman, mouse, rat, chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms TUBB3, TUJ1, beta tubulin, Tubulin beta III, Tubulin beta 3 chain
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberNM_001197181
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_2814998
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.

实验方案

Product Specific Protocols
WB protocol for TUBB3-specific/TUJ1 antibody 66375-1-IgDownload protocol
IHC protocol for TUBB3-specific/TUJ1 antibody 66375-1-IgDownload protocol
IF protocol for TUBB3-specific/TUJ1 antibody 66375-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Neuron

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Authors - Andrew Kodani
View Article
ratIF

Sci Total Environ

Ambient NO2 exposure induces migraine in rats: Evidence, mechanisms and interventions.

Authors - Shuzi Ye
View Article
ratIF

Curr Biol

Pyramidal neuron morphogenesis requires a septin network that stabilizes filopodia and suppresses lamellipodia during neurite initiation

Authors - Megan R Radler
View Article
mouseWB

Cell Rep

NLRP14 deficiency causes female infertility with oocyte maturation defects and early embryonic arrest by impairing cytoplasmic UHRF1 abundance

Authors - Wenzhe Zhang
View Article
humanIF

Theranostics

Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.

Authors - Shuai Huang
View Article
mouseWB

Carbohydr Polym

Silk fibroin nanofibrous scaffolds incorporated with microRNA-222 loaded chitosan nanoparticles for enhanced neuronal differentiation of neural stem cells.

Authors - Zehao Li
View Article