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CoraLite® Plus 488-conjugated TUBB3-specific/TUJ1 Monoclonal antibody

TUBB3-specific/TUJ1 Monoclonal Antibody for WB, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F8G10

Cat no : CL488-66375

Print datasheet

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3, tuj1, tuj1 antibody

验证数据展示

  • IF Staining of mouse spine using CL488-66375

    IF Staining of mouse spine using CL488-66375

    E14.5 FFPE mouse spine stained for beta-III tubulin (red, Cat. No CL488-66375) and GFAP (cyan, Cat. No 60190-1-Ig). Beta-III tubulin stains neurons and was conjugated to CoraLite-488 fluorescent dye and pseudocolored to red. GFAP stains astrocytes along the spinal column. In this image, astrocyte projections can be seen among spinal neurons in the spinal cord. Image credit: @Immunofluorescence on Instagram.

  • IF Staining of mouse cerebellum using CL488-66375

    IF Staining of mouse cerebellum using CL488-66375

    Neonatal mouse cerebellum stained for beta-III tubulin (magenta, Cat. No CL488-66375) and GFAP (green, Cat. No 60190-1-Ig). Beta-III tubulin stains neurons and was conjugated to CoraLite-488 fluorescent dye and pseudocolored to magenta. GFAP stains astrocytes. In this image, astrocyte projections can be seen intermingling with neurons. Image credit: @Immunofluorescence on Instagram.

  • IF Staining of mouse testis using CL488-66375

    IF Staining of mouse testis using CL488-66375

    Adult mouse testes were co-stained with TUBB3 (CL488-66375, in green), DDX4/VASA (51042-1-AP, in magenta), and PCNA (60097-1-Ig, in blue). TUBB3/Beta III tubulin marks the Sertoli cells (structural support cells), VASA marks the germ cells (developing sperm), and PCNA marks proliferating cells (these cells are at the base of the tubule, where the sperm stem cells are located). The image was created in paid partnership with @Immunofluorescence on Instagram.

  • IF Staining of mouse brain using CL488-66375

    IF Staining of mouse brain using CL488-66375

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66375 (TUBB3-specific antibody) at dilution of 1:50.

  • WB analysis using CL488-66375

    WB analysis using CL488-66375

    Neuro-2a cell lysates were subjected to SDS PAGE followed by western blot with CL488-66375 (TUBB3-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNeuro-2a cells
Positive IF-P detected inmouse brain tissue, mouse spine, mouse cerebellum tissue, mouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66375-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66375 targets TUBB3-specific/TUJ1 in WB, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3, tuj1, tuj1 antibody
Calculated Molecular Weight 55 kDa
GenBank Accession NumberNM_001197181
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_2883321
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. And the antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 TUBB3-specific/TUJ1 antibody CL488-66375Download protocol
IF protocol for CL Plus 488 TUBB3-specific/TUJ1 antibody CL488-66375Download protocol
Standard Protocols
Click here to view our Standard Protocols