CoraLite®594-conjugated TUBB3-specific/TUJ1 Monoclonal antibody
TUBB3-specific/TUJ1 Monoclonal Antibody for WB, IF-P, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IF-P, FC (Intra) and More (2)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1F8G10
验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, PC-12 cells, Neuro-2a cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66375-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
CL594-66375 targets TUBB3-specific/TUJ1 in WB, IHC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF-P, FC (Intra) Application Description |
| Cited Applications | IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | tubulin, beta 3 |
| Synonyms | beta tubulin, Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-3 chain, TUBB4 |
| Calculated Molecular Weight | 55 kDa |
| GenBank Accession Number | NM_001197181 |
| Gene Symbol | TUBB3 |
| Gene ID (NCBI) | 10381 |
| RRID | AB_2883571 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 TUBB3-specific/TUJ1 antibody CL594-66375 | Download protocol |
| IF protocol for CL594 TUBB3-specific/TUJ1 antibody CL594-66375 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Sexual dimorphic impacts of systemic vincristine on lower urinary tract function. | ||
Exp Cell Res A parasympathetic neurotransmitter induces myoepithelial cell differentiation during salivary gland development. |
