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- KD/KO Validated
XBP1S/XBP1U Polyclonal antibody
XBP1S/XBP1U Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HEK-293 cells, Jurkat cells, Raji cells |
Positive IHC detected in | human breast cancer tissue, mouse liver tissue, human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24168-1-AP targets XBP1S/XBP1U in WB, IHC, FC (Intra), ChIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, ChIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | XBP1S/XBP1U fusion protein Ag21454 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | X-box binding protein 1 |
Synonyms | XBP1, X-box-binding protein 1, luminal form, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1 |
Calculated Molecular Weight | 261 aa, 29 kDa |
Observed Molecular Weight | ~32 kDa; ~60 kDa |
GenBank Accession Number | BC000938 |
Gene Symbol | XBP1 |
Gene ID (NCBI) | 7494 |
RRID | AB_2879445 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
UNIPROT ID | P17861 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for XBP1S/XBP1U antibody 24168-1-AP | Download protocol |
IHC protocol for XBP1S/XBP1U antibody 24168-1-AP | Download protocol |
FC protocol for XBP1S/XBP1U antibody 24168-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Ethnopharmacol Network pharmacology, molecular docking, and in vitro experimental verification of the mechanism of Guanxining in treating diabetic atherosclerosis | ||
Bioorg Chem Dihydroartemisinin alleviates steatosis and inflammation in nonalcoholic steatohepatitis by decreasing endoplasmic reticulum stress and oxidative stress. | ||
J Virol Inositol requiring enzyme 1α promotes Zika virus infection through regulating stearoyl-CoA desaturase 1 mediated lipid metabolism.
| ||
J Zhejiang Univ Sci B Endoplasmic reticulum stress is involved in retinal injury induced by repeated transient spikes of intraocular pressure | ||
Cell Death Discov 24(S)-Hydroxycholesterol induces ER dysfunction-mediated unconventional cell death. | ||
J Exp Clin Cancer Res Calreticulin promotes EMT in pancreatic cancer via mediating Ca2+ dependent acute and chronic endoplasmic reticulum stress. |