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CoraLite® Plus 488-conjugated XBP1S/XBP1U Polyclonal antibody

XBP1S/XBP1U Polyclonal Antibody for FC (Intra)
Cat No. CL488-24168

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

XBP1, X-box-binding protein 1, luminal form, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 24168-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24168 targets XBP1S/XBP1U in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 XBP1S/XBP1U fusion protein Ag21454 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 X-box binding protein 1
别名 XBP1, X-box-binding protein 1, luminal form, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1
计算分子量 261 aa, 29 kDa
观测分子量 ~32 kDa; ~60 kDa
GenBank蛋白编号BC000938
基因名称 XBP1
Gene ID (NCBI) 7494
RRIDAB_3672760
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17861
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XBP1 is also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). IRE1α possesses both kinase and ribonuclease activity and processes XBP1 mRNA to produce an active transcription factor in response to ER stress (PMID: 11779464, 11780124). It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum, the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1. The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1S, which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1U, is constitutively expressed, and thought to function as a negative feedback regulator of XBP1S, which shuts off transcription of target genes during the recovery phase of ER stress (PMID: 11850408). The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The XBP1 antibody (24168-1-AP) can detect both XBP1U and XBP1S.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 XBP1S/XBP1U antibody CL488-24168Download protocol
Standard Protocols
Click here to view our Standard Protocols
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