pan-keratin Polyclonal antibody

pan-keratin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 26411-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

pan-CK, CK 5, Cytokeratin 5, CK-5, keratin 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells, HeLa cells
Positive IHC detected inhuman oesophagus tissue, human brown disease, human cervical cancer tissue, human liver tissue, human lung cancer tissue, human renal cell carcinoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue, human rectal cancer tissue
Positive IF/ICC detected inA431 cells, HeLa cells, mouse breast cancer
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:50000-1:200000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26411-1-AP targets pan-keratin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, sheep
免疫原 pan-keratin fusion protein Ag24184 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 5
别名 pan-CK, CK 5, Cytokeratin 5, CK-5, keratin 5
计算分子量 590 aa, 62 kDa
观测分子量 46-58 kDa
GenBank蛋白编号BC024292
基因名称 Cytokeratin 5
Gene ID (NCBI) 3852
RRIDAB_2880505
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13647
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pan-keratin, also known as pan-cytokeratin, refers to a group of antibodies that recognize a broad array of keratin proteins. Keratins are a large family of intermediate filament (IF) proteins that provide structural integrity to epithelial cells. These proteins are essential for maintaining cell shape, strength, and resilience . The keratin gene family is divided into two major groups: type I keratins (acidic) and type II keratins (basic), with 27 type I and 26 type II keratin genes in humans. Pan-keratin immunostaining is widely used in pathology and research due to its ability to detect a variety of keratin proteins, making it a valuable tool for identifying epithelial cells and tissues. This staining technique is particularly useful in the diagnosis of tumors, as it can help distinguish between epithelial and non-epithelial neoplasms. This antibody is a pan-keratin antibody.

实验方案

Product Specific Protocols
WB protocol for pan-keratin antibody 26411-1-APDownload protocol
IHC protocol for pan-keratin antibody 26411-1-APDownload protocol
IF protocol for pan-keratin antibody 26411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

The estrogen response in fibroblasts promotes ovarian metastases of gastric cancer

Authors - Simeng Hu
humanIF

Biomaterials

Heterotypic 3D pancreatic cancer model with tunable proportion of fibrotic elements.

Authors - Hiroyoshi Y Tanaka
mouseIF

Biomaterials

MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.

Authors - Tian Wang
IHC

J Nanobiotechnology

ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis

Authors - Xuan Lai
humanIF

Oncogene

The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer

Authors - Mengjie Li
mouseIHC

Oncogene

USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.

Authors - Juntae Kwon
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