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CoraLite® Plus 488-conjugated pan-keratin Polyclonal antibody

pan-keratin Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26411

Print datasheet

Synonyms

CK 5, Cytokeratin 5, CK-5, keratin 5, KRT5

验证数据展示

  • IF Staining of human colon cancer using CL488-26411

    IF Staining of human colon cancer using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

  • IF Staining of HeLa using CL488-26411

    IF Staining of HeLa using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:100.

  • IF Staining of human lung cancer using CL488-26411

    IF Staining of human lung cancer using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD20 antibody (CL647-60271, Clone: 4A7G3, Magenta).

  • IF Staining of human colon cancer using CL488-26411

    IF Staining of human colon cancer using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:100.

  • IF Staining of human colon cancer using CL488-26411

    IF Staining of human colon cancer using CL488-26411

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:100.

  • FC experiment of A431 using CL488-26411

    FC experiment of A431 using CL488-26411

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human pan-keratin (CL488-26411) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inhuman colon cancer tissue, human lung cancer tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 26411-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26411 targets pan-keratin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity human
Immunogen pan-keratin fusion protein Ag24184 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 5
Synonyms CK 5, Cytokeratin 5, CK-5, keratin 5, KRT5
Calculated Molecular Weight 590 aa, 62 kDa
GenBank Accession NumberBC024292
Gene Symbol Cytokeratin 5
Gene ID (NCBI) 3852
RRIDAB_2919208
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13647
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 pan-keratin antibody CL488-26411Download protocol
FC protocol for CL Plus 488 pan-keratin antibody CL488-26411Download protocol
Standard Protocols
Click here to view our Standard Protocols