验证数据展示
经过测试的应用
Positive IF-P detected in | human colon cancer tissue, human lung cancer tissue, human rectal cancer tissue |
Positive IF detected in | human colon cancer tissue |
Positive FC (Intra) detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-26411 targets pan-keratin in IF, IF-P, FC (Intra) applications and shows reactivity with human samples.
经测试应用 | IF, IF-P, FC (Intra) Application Description |
经测试反应性 | human |
免疫原 | pan-keratin fusion protein Ag24184 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | keratin 5 |
别名 | CK 5, Cytokeratin 5, CK-5, keratin 5, KRT5 |
计算分子量 | 590 aa, 62 kDa |
GenBank蛋白编号 | BC024292 |
基因名称 | Cytokeratin 5 |
Gene ID (NCBI) | 3852 |
RRID | AB_2919208 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P13647 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 pan-keratin antibody CL488-26411 | Download protocol |
FC protocol for CL Plus 488 pan-keratin antibody CL488-26411 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |