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验证数据展示

  • FC experiment of A431 using CL647-26411

    FC experiment of A431 using CL647-26411

    1x10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated pan-keratin Polyclonal antibody (CL647-26411)(red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated pan-keratin Polyclonal antibody

pan-keratin Polyclonal Antibody for FC (Intra)
Cat No. CL647-26411

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

58 kDa cytokeratin, CK 5, CK5, Cytokeratin 5, K5, keratin 5, KRT5, KRT5A, pan Cytokeratin, pan-ck, pan-cytokeratin, pan-keratin, Type II keratin Kb5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 26411-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-26411 targets pan-keratin in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 pan-keratin fusion protein Ag24184 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 5
别名 58 kDa cytokeratin, CK 5, CK5, Cytokeratin 5, K5, keratin 5, KRT5, KRT5A, pan Cytokeratin, pan-ck, pan-cytokeratin, pan-keratin, Type II keratin Kb5
计算分子量 590 aa, 62 kDa
观测分子量 46-58 kDa
GenBank蛋白编号BC024292
基因名称 Cytokeratin 5
Gene ID (NCBI) 3852
RRIDAB_2934961
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13647
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 pan-keratin antibody CL647-26411Download protocol
Standard Protocols
Click here to view our Standard Protocols