- Featured Product
- KD/KO Validated
KI67 Polyclonal antibody
KI67 Polyclonal Antibody for FC, IF/ICC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
FC, IF/ICC, IHC, ELISA
Conjugate
Unconjugated
866
验证数据展示
经过测试的应用
Positive IHC detected in | human tonsillitis tissue, K-562 cells, Insulinoma tissue, human lung cancer tissue, human breast cancer tissue, human skin cancer tissue, human lymphoma tissue, human colon cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, HEK-293 cells |
Positive FC detected in | Jurkat cells |
推荐稀释比
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:2000-1:10000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27309-1-AP targets KI67 in IF, FC, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IF/ICC, IHC, ELISA |
Cited Applications | IF, FC, IHC |
Tested Reactivity | human |
Cited Reactivity | human, goat, rabbit, hamster, pig |
Immunogen | KI67 fusion protein Ag26266 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | antigen identified by monoclonal antibody Ki-67 |
Synonyms | Antigen KI 67, Ki 67, KI67, KIA, MKI67 |
Calculated Molecular Weight | 359 kDa |
GenBank Accession Number | NM_002417 |
Gene Symbol | MKI67 |
Gene ID (NCBI) | 4288 |
RRID | AB_2756525 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P46013 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IHC protocol for KI67 antibody 27309-1-AP | Download protocol |
IF protocol for KI67 antibody 27309-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth | ||
Mol Cancer lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2 | ||
Cancer Discov Stress Granules determine the Development of Obesity-associated Pancreatic Cancer. | ||
Gastroenterology Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6. | ||
ACS Nano Enzyme-Triggered Transcytosis of Dendrimer-Drug Conjugate for Deep Penetration into Pancreatic Tumors. | ||
Blood C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse |