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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated Ki-67 Polyclonal antibody

Ki-67 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-27309

Print datasheet

Synonyms

MKI67, KI67, Proliferation marker protein Ki-67, Ki 67, Antigen KI-67

验证数据展示

  • IF Staining of HeLa using CL555-27309

    IF Staining of HeLa using CL555-27309

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 KI67 antibody (CL555-27309) at dilution of 1:200.

  • IF Staining of HeLa using CL555-27309

    IF Staining of HeLa using CL555-27309

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 KI67 antibody (CL555-27309) at dilution of 1:200.

  • FC experiment of Jurkat using CL555-27309

    FC experiment of Jurkat using CL555-27309

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human KI67 (CL555-27309) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 27309-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-27309 targets Ki-67 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen Ki-67 fusion protein Ag26266 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name antigen identified by monoclonal antibody Ki-67
Synonyms MKI67, KI67, Proliferation marker protein Ki-67, Ki 67, Antigen KI-67
Calculated Molecular Weight 359 kDa
GenBank Accession NumberNM_002417
Gene Symbol KI67
Gene ID (NCBI) 4288
RRIDAB_2919649
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

实验方案

Product Specific Protocols
IF protocol for CL555 Ki-67 antibody CL555-27309Download protocol
Standard Protocols
Click here to view our Standard Protocols