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验证数据展示

  • IF Staining of HeLa using CL555-27309

    IF Staining of HeLa using CL555-27309

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 KI67 antibody (CL555-27309) at dilution of 1:200.

  • IF Staining of HeLa using CL555-27309

    IF Staining of HeLa using CL555-27309

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 KI67 antibody (CL555-27309) at dilution of 1:200.

  • FC experiment of Jurkat using CL555-27309

    FC experiment of Jurkat using CL555-27309

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human KI67 (CL555-27309) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated Ki-67 Polyclonal antibody

Ki-67 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-27309

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

MKI67, KI67, Proliferation marker protein Ki-67, Ki 67, Antigen KI-67

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy KI67 Ready-To-Use IHC Kit. KI67 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27309-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-27309 targets Ki-67 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Ki-67 fusion protein Ag26266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 antigen identified by monoclonal antibody Ki-67
别名 MKI67, KI67, Proliferation marker protein Ki-67, Ki 67, Antigen KI-67
计算分子量 359 kDa
GenBank蛋白编号NM_002417
基因名称 KI67
Gene ID (NCBI) 4288
RRIDAB_2919649
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

实验方案

Product Specific Protocols
IF protocol for CL555 Ki-67 antibody CL555-27309Download protocol
Standard Protocols
Click here to view our Standard Protocols