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CoraLite®488-conjugated KI67 Polyclonal antibody

KI67 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), IF

Conjugate

CoraLite®488 Fluorescent Dye

Cat No : CL488-27309

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Synonyms

Antigen KI 67, Ki 67, KI67, KIA, MKI67



经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 27309-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of CL488-27309 is KI67 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), IF
Tested Reactivity human
Immunogen KI67 fusion protein Ag26266 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name antigen identified by monoclonal antibody Ki-67
Synonyms Antigen KI 67, Ki 67, KI67, KIA, MKI67
Calculated molecular weight 359 kDa
GenBank accession numberNM_002417
Gene symbol MKI67
Gene ID (NCBI) 4288
RRIDAB_2919213
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

实验方案

Product Specific Protocols
IF protocol for CL488 KI67 antibody CL488-27309Download protocol
Standard Protocols
Click here to view our Standard Protocols