验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, pig liver tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-87 MG cells, HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 4 publications below |
| IP | See 1 publications below |
产品信息
60062-1-Ig targets GMF Beta in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF, IP |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 | GMF Beta fusion protein Ag1104 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glia maturation factor, beta |
| 别名 | glia maturation factor beta, GMF Beta, GMFB, GMF-beta |
| 计算分子量 | 17 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC005359 |
| 基因名称 | GMFB |
| Gene ID (NCBI) | 2764 |
| RRID | AB_2110835 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P60983 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GMF Beta antibody 60062-1-Ig | Download protocol |
| IHC protocol for GMF Beta antibody 60062-1-Ig | Download protocol |
| IF protocol for GMF Beta antibody 60062-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol SUMOylation of GMFB regulates its stability and function in retinal pigment epithelial cells under hyperglycemia | ||
Transl Psychiatry Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein. | ||
Front Oncol Glia Maturation Factor β as a Novel Independent Prognostic Biomarker and Potential Therapeutic Target of Kidney Renal Clear Cell Carcinoma. | ||
J Alzheimers Dis Co-Localization of Glia Maturation Factor with NLRP3 Inflammasome and Autophagosome Markers in Human Alzheimer's Disease Brain. | ||
PLoS One Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators. |
