验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, Jurkat cells, U2OS cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, A431 cells, LNCaP cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas tissue, human breast hyperplasia tissue, human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60265-1-Ig targets SND1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | SND1 fusion protein Ag1200 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | staphylococcal nuclease and tudor domain containing 1 |
| 别名 | 100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11 |
| 计算分子量 | 101 kDa |
| 观测分子量 | 101 kDa |
| GenBank蛋白编号 | BC017180 |
| 基因名称 | SND1 |
| Gene ID (NCBI) | 27044 |
| RRID | AB_2881386 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q7KZF4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SND1 antibody 60265-1-Ig | Download protocol |
| IHC protocol for SND1 antibody 60265-1-Ig | Download protocol |
| IF protocol for SND1 antibody 60265-1-Ig | Download protocol |
| IP protocol for SND1 antibody 60265-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9 | ||
Sci Adv Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor. | ||
Sci Adv Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor. | ||
Elife The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.
| ||
J Cell Physiol The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs. |
