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验证数据展示

  • WB analysis using 60295-1-Ig

    WB analysis using 60295-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60295-1-Ig (SCGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of human lymphoma using 60295-1-Ig

    IF Staining of human lymphoma using 60295-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human lymphoma using 60295-1-Ig

    IHC staining of human lymphoma using 60295-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lymphoma using 60295-1-Ig

    IHC staining of human lymphoma using 60295-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 60295-1-Ig

    IF Staining of HepG2 using 60295-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

SCGF Monoclonal antibody

SCGF Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 60295-1-Ig

产品说明书

CloneNo. 4H11E4
引用文献(3)

宿主/亚型

Mouse / IgG2b

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

别名

CLEC11A, 4H11E4, LSLCL, C-type lectin superfamily member 3, C-type lectin domain family 11 member A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, human heart tissue, Kasumi-1 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lymphoma tissue, HepG2 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60295-1-Ig targets SCGF in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 SCGF fusion protein Ag21365 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 C-type lectin domain family 11, member A
别名 CLEC11A, 4H11E4, LSLCL, C-type lectin superfamily member 3, C-type lectin domain family 11 member A
计算分子量 36 kDa
观测分子量36-40 kDa
GenBank蛋白编号BC005810
基因名称 SCGF
Gene ID (NCBI) 6320
RRIDAB_2881410
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y240
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLEC11A, also named as CLECSF3, LSLCL, SCGF and p47, stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. CLEC11A acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. It suppresses SCF-stimulated erythrocyte proliferation.

实验方案

Product Specific Protocols
WB protocol for SCGF antibody 60295-1-IgDownload protocol
IHC protocol for SCGF antibody 60295-1-IgDownload protocol
IF protocol for SCGF antibody 60295-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC, IF

FASEB J

The "Matrisome" reveals the characterization of skin keloid microenvironment.

Authors - Shikun Zhang
View Article
mouseWB

Stem Cell Rev Rep

Daidzein Activates Akt Pathway to Promote the Proliferation of Female Germline Stem Cells through Upregulating Clec11a.

Authors - Fangfang Li
View Article
humanWB

Biomol Biomed

CLEC11A expression as a prognostic biomarker in correlation to immune cells of gastric cancer

Authors - Weidan Fang
View Article