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验证数据展示

  • IF Staining of HepG2 using CL488-60295

    IF Staining of HepG2 using CL488-60295

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 SCGF antibody (CL488-60295, Clone: 4H11E4 ) at dilution of 1:200, CL594-phalloidin (red).

CoraLite® Plus 488-conjugated SCGF Monoclonal antibody

SCGF Monoclonal Antibody for IF/ICC
Cat No. CL488-60295

产品说明书

CloneNo. 4H11E4

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC

别名

CLEC11A, CLECSF3, LSLCL, P47, SCGF, Stem cell growth factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60295-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60295 targets SCGF in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 SCGF fusion protein Ag21365 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 C-type lectin domain family 11, member A
别名 CLEC11A, CLECSF3, LSLCL, P47, SCGF, Stem cell growth factor
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC005810
基因名称 SCGF
Gene ID (NCBI) 6320
RRIDAB_3084146
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y240
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLEC11A, also named as CLECSF3, LSLCL, SCGF and p47, stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. CLEC11A acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. It suppresses SCF-stimulated erythrocyte proliferation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SCGF antibody CL488-60295Download protocol
Standard Protocols
Click here to view our Standard Protocols