验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, human heart tissue, Kasumi-1 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lymphoma tissue, HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60295-1-Ig targets SCGF in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | SCGF fusion protein Ag21365 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | C-type lectin domain family 11, member A |
| Synonyms | CLEC11A, 4H11E4, LSLCL, C-type lectin superfamily member 3, C-type lectin domain family 11 member A |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36-40 kDa |
| GenBank Accession Number | BC005810 |
| Gene Symbol | SCGF |
| Gene ID (NCBI) | 6320 |
| RRID | AB_2881410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y240 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CLEC11A, also named as CLECSF3, LSLCL, SCGF and p47, stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. CLEC11A acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. It suppresses SCF-stimulated erythrocyte proliferation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SCGF antibody 60295-1-Ig | Download protocol |
| IHC protocol for SCGF antibody 60295-1-Ig | Download protocol |
| IF protocol for SCGF antibody 60295-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cell Rev Rep Daidzein Activates Akt Pathway to Promote the Proliferation of Female Germline Stem Cells through Upregulating Clec11a. | ||
Biomol Biomed CLEC11A expression as a prognostic biomarker in correlation to immune cells of gastric cancer |
