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SCGF Monoclonal antibody, PBS Only

SCGF Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H11E4

Cat no : 60295-1-PBS

Print datasheet

Synonyms

CLEC11A, 4H11E4, LSLCL, C-type lectin superfamily member 3, C-type lectin domain family 11 member A

验证数据展示

  • WB analysis using 60295-1-Ig (same clone as 60295-1-PBS)

    WB analysis using 60295-1-Ig (same clone as 60295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60295-1-Ig (SCGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

  • IF Staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

    IF Staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

    IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

    IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60295-1-Ig (same clone as 60295-1-PBS)

    IF Staining of HepG2 using 60295-1-Ig (same clone as 60295-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

产品信息

60295-1-PBS targets SCGF in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen SCGF fusion protein Ag21365 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name C-type lectin domain family 11, member A
Synonyms CLEC11A, 4H11E4, LSLCL, C-type lectin superfamily member 3, C-type lectin domain family 11 member A
Calculated Molecular Weight 36 kDa
Observed Molecular Weight36-40 kDa
GenBank Accession NumberBC005810
Gene Symbol SCGF
Gene ID (NCBI) 6320
RRIDAB_2881410
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y240
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CLEC11A, also named as CLECSF3, LSLCL, SCGF and p47, stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. CLEC11A acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. It suppresses SCF-stimulated erythrocyte proliferation.