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IA-2/PTPRN Monoclonal antibody

IA-2/PTPRN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3F5

Cat no : 66045-1-Ig

Print datasheet

Synonyms

IA 2/PTP, IA2, IA-2, ICA 512, ICA3, ICA512, Islet cell antigen 512, Islet cell autoantigen 3, PTP IA 2, PTPRN, R PTP N

验证数据展示

  • WB analysis of human brain using 66045-1-Ig

    WB analysis of human brain using 66045-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 66045-1-Ig

    IHC staining of human pancreas cancer using 66045-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPRN antibody included
  • IHC staining of human pancreas cancer using 66045-1-Ig

    IHC staining of human pancreas cancer using 66045-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPRN antibody included
  • IF Staining of HepG2 using 66045-1-Ig

    IF Staining of HepG2 using 66045-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTPRN Ready-To-Use IHC Kit. PTPRN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66045-1-Ig targets IA-2/PTPRN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen IA-2/PTPRN fusion protein Ag17909 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, N
Synonyms IA 2/PTP, IA2, IA-2, ICA 512, ICA3, ICA512, Islet cell antigen 512, Islet cell autoantigen 3, PTP IA 2, PTPRN, R PTP N
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 60-65 kDa, 106-115 kDa
GenBank Accession NumberBC007713
Gene Symbol IA-2/PTPRN
Gene ID (NCBI) 5798
RRIDAB_11064603
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16849
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

实验方案

Product Specific Protocols
WB protocol for IA-2/PTPRN antibody 66045-1-IgDownload protocol
IHC protocol for IA-2/PTPRN antibody 66045-1-IgDownload protocol
IF protocol for IA-2/PTPRN antibody 66045-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols