• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human liver cancer using 66052-1-Ig

    IHC staining of human liver cancer using 66052-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINC1 antibody included
  • IHC staining of human liver cancer using 66052-1-Ig

    IHC staining of human liver cancer using 66052-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINC1 antibody included
  • WB analysis of human plasma using 66052-1-Ig

    WB analysis of human plasma using 66052-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Serpin C1/Antithrombin III antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 66052-1-Ig

    WB analysis of L02 using 66052-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66052-1-Ig

    IF Staining of HepG2 using 66052-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Serpin C1/Antithrombin III antibody (66052-1-Ig, Clone: 2B2E5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • IF Staining of L02 using 66052-1-Ig

    IF Staining of L02 using 66052-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed L02 cells using 66052-1-Ig(Antithrombin III antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Serpin C1/Antithrombin III Monoclonal antibody

Serpin C1/Antithrombin III Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66052-1-Ig

产品说明书

CloneNo. 2B2E5
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Antithrombin III, Serpin C1, SERPINC1, 2B2E5, PRO0309

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman plasma, human brain tissue, human testis tissue, L02 cells, rat brain tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, L02 cells
Planning an IHC experiment? We recommend our IHCeasy SERPINC1 Ready-To-Use IHC Kit. SERPINC1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66052-1-Ig targets Serpin C1/Antithrombin III in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Serpin C1/Antithrombin III fusion protein Ag9880 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade C (antithrombin), member 1
别名 Antithrombin III, Serpin C1, SERPINC1, 2B2E5, PRO0309
计算分子量 464 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC022309
基因名称 Serpin C1/Antithrombin III
Gene ID (NCBI) 462
RRIDAB_11042589
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01008
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

实验方案

Product Specific Protocols
WB protocol for Serpin C1/Antithrombin III antibody 66052-1-IgDownload protocol
IHC protocol for Serpin C1/Antithrombin III antibody 66052-1-IgDownload protocol
IF protocol for Serpin C1/Antithrombin III antibody 66052-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
View Article
mouseWB

Front Oncol

Synergistic effects of nab-PTX and anti-PD-1 antibody combination against lung cancer by regulating the Pi3K/AKT pathway through the Serpinc1 gene

Authors - Jun Zhang
View Article