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Serpin C1/Antithrombin III Monoclonal antibody, PBS Only

Serpin C1/Antithrombin III Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B2E5

Cat no : 66052-1-PBS

Print datasheet

Synonyms

Antithrombin III, Serpin C1, SERPINC1, 2B2E5, PRO0309

验证数据展示

  • IHC staining of human liver cancer using 66052-1-Ig (same clone as 66052-1-PBS)

    IHC staining of human liver cancer using 66052-1-Ig (same clone as 66052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SERPINC1 antibody included
  • IHC staining of human liver cancer using 66052-1-Ig (same clone as 66052-1-PBS)

    IHC staining of human liver cancer using 66052-1-Ig (same clone as 66052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SERPINC1 antibody included
  • WB analysis of human plasma using 66052-1-Ig (same clone as 66052-1-PBS)

    WB analysis of human plasma using 66052-1-Ig (same clone as 66052-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Serpin C1/Antithrombin III antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • WB analysis of L02 using 66052-1-Ig (same clone as 66052-1-PBS)

    WB analysis of L02 using 66052-1-Ig (same clone as 66052-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66052-1-Ig (same clone as 66052-1-PBS)

    IF Staining of HepG2 using 66052-1-Ig (same clone as 66052-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Serpin C1/Antithrombin III antibody (66052-1-Ig, Clone: 2B2E5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • IF Staining of L02 using 66052-1-Ig (same clone as 66052-1-PBS)

    IF Staining of L02 using 66052-1-Ig (same clone as 66052-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed L02 cells using 66052-1-Ig(Antithrombin III antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SERPINC1 Ready-To-Use IHC Kit. SERPINC1 primary antibody included.

产品信息

66052-1-PBS targets Serpin C1/Antithrombin III in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Serpin C1/Antithrombin III fusion protein Ag9880 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade C (antithrombin), member 1
Synonyms Antithrombin III, Serpin C1, SERPINC1, 2B2E5, PRO0309
Calculated Molecular Weight 464 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC022309
Gene Symbol Serpin C1/Antithrombin III
Gene ID (NCBI) 462
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01008
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.