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Serpin C1/Antithrombin III Monoclonal antibody

Serpin C1/Antithrombin III Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2B2E5

Cat no : 66052-1-Ig

Print datasheet

Synonyms

Antithrombin III, Serpin C1, SERPINC1, 2B2E5, PRO0309

验证数据展示

  • IHC staining of human liver cancer using 66052-1-Ig

    IHC staining of human liver cancer using 66052-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINC1 antibody included
  • IHC staining of human liver cancer using 66052-1-Ig

    IHC staining of human liver cancer using 66052-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SERPINC1 antibody included
  • WB analysis of human plasma using 66052-1-Ig

    WB analysis of human plasma using 66052-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Serpin C1/Antithrombin III antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 66052-1-Ig

    WB analysis of L02 using 66052-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66052-1-Ig

    IF Staining of HepG2 using 66052-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Serpin C1/Antithrombin III antibody (66052-1-Ig, Clone: 2B2E5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • IF Staining of L02 using 66052-1-Ig

    IF Staining of L02 using 66052-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed L02 cells using 66052-1-Ig(Antithrombin III antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma, human brain tissue, human testis tissue, L02 cells, rat brain tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, L02 cells
Planning an IHC experiment? We recommend our IHCeasy SERPINC1 Ready-To-Use IHC Kit. SERPINC1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66052-1-Ig targets Serpin C1/Antithrombin III in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Serpin C1/Antithrombin III fusion protein Ag9880 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade C (antithrombin), member 1
Synonyms Antithrombin III, Serpin C1, SERPINC1, 2B2E5, PRO0309
Calculated Molecular Weight 464 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC022309
Gene Symbol Serpin C1/Antithrombin III
Gene ID (NCBI) 462
RRIDAB_11042589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01008
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

实验方案

Product Specific Protocols
WB protocol for Serpin C1/Antithrombin III antibody 66052-1-IgDownload protocol
IHC protocol for Serpin C1/Antithrombin III antibody 66052-1-IgDownload protocol
IF protocol for Serpin C1/Antithrombin III antibody 66052-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
View Article
mouseWB

Front Oncol

Synergistic effects of nab-PTX and anti-PD-1 antibody combination against lung cancer by regulating the Pi3K/AKT pathway through the Serpinc1 gene

Authors - Jun Zhang
View Article