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验证数据展示

  • IF Staining of HepG2 using CL488-66052

    IF Staining of HepG2 using CL488-66052

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Serpin C1/Antithrombin III antibody (CL488-66052, Clone: 2B2E5 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Serpin C1/Antithrombin III Monoclonal antibody

Serpin C1/Antithrombin III Monoclonal Antibody for IF/ICC
Cat No. CL488-66052
CloneNo. 2B2E5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

Antithrombin, Antithrombin III, AT3, ATIII, HEPARIN COFACTOR I, Serpin C1, SERPINC1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SERPINC1 Ready-To-Use IHC Kit. SERPINC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66052-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66052 targets Serpin C1/Antithrombin III in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Serpin C1/Antithrombin III fusion protein Ag9880 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade C (antithrombin), member 1
Synonyms Antithrombin, Antithrombin III, AT3, ATIII, HEPARIN COFACTOR I, Serpin C1, SERPINC1
Calculated Molecular Weight 464 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC022309
Gene Symbol Serpin C1/Antithrombin III
Gene ID (NCBI) 462
RRIDAB_3084201
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP01008
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Serpin C1/Antithrombin III antibody CL488-66052Download protocol
Standard Protocols
Click here to view our Standard Protocols