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验证数据展示

  • WB analysis of HepG2 using 66053-1-Ig (same clone as 66053-1-PBS)

    WB analysis of HepG2 using 66053-1-Ig (same clone as 66053-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

    IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

    IHC staining of human kidney using 66053-1-Ig (same clone as 66053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66053-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50607-1

    Cytometric bead array standard curve of MP50607-1

    Cytometric bead array standard curve of MP50607-1, NDUFS5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66053-2-PBS. Detection antibody: 66053-1-PBS. Standard:Ag7663. Range: 3.125-100 ng/mL.

NDUFS5 Monoclonal antibody, PBS Only (Detector)

NDUFS5 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66053-1-PBS

产品说明书

CloneNo. 2C2A5

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, Complex I-15 kDa, Complex I 15 kDa, CI-15 kDa, CI 15 kDa

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66053-1-PBS targets NDUFS5 as part of a matched antibody pair:

MP50607-1: 66053-2-PBS capture and 66053-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 NDUFS5 fusion protein Ag7663 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
别名 NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, Complex I-15 kDa, Complex I 15 kDa, CI-15 kDa, CI 15 kDa
计算分子量 13 kDa
观测分子量 15 kDa
GenBank蛋白编号BC001884
基因名称 NDUFS5
Gene ID (NCBI) 4725
RRIDAB_11043342
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43920
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150). Proteinteach's NDUFS5 antibody 66053-1-Ig is capable of detecting the 15 kDa protein in Western blotting.