验证数据展示
经过测试的应用
| Positive WB detected in | human plasma, human testis tissue, pig plasma, human heart tissue, pig heart tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy APOH Ready-To-Use IHC Kit. APOH primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
66074-1-Ig targets APOH in WB, IHC, IF/ICC, IF-P, Neutralization, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, Neutralization |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 | APOH fusion protein Ag17915 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | apolipoprotein H (beta-2-glycoprotein I) |
| 别名 | Apolipoprotein H, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein |
| 计算分子量 | 345 aa, 38 kDa |
| 观测分子量 | 50-53 kDa |
| GenBank蛋白编号 | BC026283 |
| 基因名称 | APOH |
| Gene ID (NCBI) | 350 |
| RRID | AB_11182392 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P02749 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APOH antibody 66074-1-Ig | Download protocol |
| IHC protocol for APOH antibody 66074-1-Ig | Download protocol |
| IF protocol for APOH antibody 66074-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Thromb Haemost Identification of APOH polymorphisms as common genetic risk factors for venous thrombosis in the Chinese population. | ||
Curr Med Sci Thrombophilia Caused by Beta2-Glycoprotein I Deficiency: In Vitro Study of a Rare Mutation in APOH Gene. | ||
Biomedicines Identification of Potential Drug Targets for Antiplatelet Therapy Specifically Targeting Platelets of Old Individuals through Proteomic Analysis | ||
Cell Death Dis Serum apolipoprotein H determines ferroptosis resistance by modulating cellular lipid composition |
