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Apolipoprotein H Monoclonal antibody, PBS Only

Apolipoprotein H Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4D9A4

Cat no : 66074-1-PBS

Print datasheet

Synonyms

APOH, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein

验证数据展示

  • IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOH antibody included
  • WB analysis using 66074-1-Ig (same clone as 66074-1-PBS)

    WB analysis using 66074-1-Ig (same clone as 66074-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOH antibody included
  • IF Staining of HepG2 using 66074-1-Ig (same clone as 66074-1-PBS)

    IF Staining of HepG2 using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein H antibody (66074-1-Ig, Clone: 4D9A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    IF Staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Apolipoprotein H antibody (66074-1-Ig, Clone: 4D9A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy APOH Ready-To-Use IHC Kit. APOH primary antibody included.

产品信息

66074-1-PBS targets Apolipoprotein H in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Apolipoprotein H fusion protein Ag17915 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name apolipoprotein H (beta-2-glycoprotein I)
Synonyms APOH, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein
Calculated Molecular Weight 345 aa, 38 kDa
Observed Molecular Weight 50-53 kDa
GenBank Accession NumberBC026283
Gene Symbol APOH
Gene ID (NCBI) 350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02749
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.