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验证数据展示

  • IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOH antibody included
  • WB analysis using 66074-1-Ig (same clone as 66074-1-PBS)

    WB analysis using 66074-1-Ig (same clone as 66074-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with APOH antibody included
  • IF Staining of HepG2 using 66074-1-Ig (same clone as 66074-1-PBS)

    IF Staining of HepG2 using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein H antibody (66074-1-Ig, Clone: 4D9A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    IF Staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Apolipoprotein H antibody (66074-1-Ig, Clone: 4D9A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

APOH Monoclonal antibody, PBS Only

APOH Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66074-1-PBS

产品说明书

CloneNo. 4D9A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

Apolipoprotein H, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy APOH Ready-To-Use IHC Kit. APOH primary antibody included.

产品信息

66074-1-PBS targets APOH in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 APOH fusion protein Ag17915 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 apolipoprotein H (beta-2-glycoprotein I)
别名 Apolipoprotein H, ApolipoproteinH, Apo-H, Apo H, Activated protein C-binding protein
计算分子量 345 aa, 38 kDa
观测分子量 50-53 kDa
GenBank蛋白编号BC026283
基因名称 APOH
Gene ID (NCBI) 350
RRIDAB_11182392
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02749
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.