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验证数据展示

  • WB analysis of human placenta using 66112-1-Ig

    WB analysis of human placenta using 66112-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66112-1-Ig

    IHC staining of human liver using 66112-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50.

  • IHC staining of human liver using 66112-1-Ig

    IHC staining of human liver using 66112-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50.

  • IF Staining of HepG2 using 66112-1-Ig

    IF Staining of HepG2 using 66112-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Iduronate 2 sulfatase antibody (66112-1-Ig, Clone: 1A3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

Iduronate 2 sulfatase Monoclonal antibody

Iduronate 2 sulfatase Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66112-1-Ig

产品说明书

CloneNo. 1A3F9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

IDS, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase, EC:3.1.6.13, Alpha-L-iduronate sulfate sulfatase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHuman placenta, rat brain tissue, human brain tissue, mouse brain tissue, pig brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66112-1-Ig targets Iduronate 2 sulfatase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Iduronate 2 sulfatase fusion protein Ag19095 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 iduronate 2-sulfatase
别名 IDS, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase, EC:3.1.6.13, Alpha-L-iduronate sulfate sulfatase
计算分子量312aa,35 kDa; 550aa,62 kDa
观测分子量 76 kDa, 55 kDa
GenBank蛋白编号BC006170
基因名称 IDS
Gene ID (NCBI) 3423
RRIDAB_2881511
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP22304
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).

实验方案

Product Specific Protocols
WB protocol for Iduronate 2 sulfatase antibody 66112-1-IgDownload protocol
IHC protocol for Iduronate 2 sulfatase antibody 66112-1-IgDownload protocol
IF protocol for Iduronate 2 sulfatase antibody 66112-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols