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Iduronate 2 sulfatase Monoclonal antibody, PBS Only

Iduronate 2 sulfatase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A3F9

Cat no : 66112-1-PBS

Print datasheet

Synonyms

IDS, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase, EC:3.1.6.13, Alpha-L-iduronate sulfate sulfatase

验证数据展示

  • WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

    WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

    IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

    IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66112-1-Ig (same clone as 66112-1-PBS)

    IF Staining of HepG2 using 66112-1-Ig (same clone as 66112-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Iduronate 2 sulfatase antibody (66112-1-Ig, Clone: 1A3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

产品信息

66112-1-PBS targets Iduronate 2 sulfatase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Iduronate 2 sulfatase fusion protein Ag19095 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name iduronate 2-sulfatase
Synonyms IDS, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase, EC:3.1.6.13, Alpha-L-iduronate sulfate sulfatase
Calculated Molecular Weight312aa,35 kDa; 550aa,62 kDa
Observed Molecular Weight 76 kDa, 55 kDa
GenBank Accession NumberBC006170
Gene Symbol IDS
Gene ID (NCBI) 3423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22304
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).