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Iduronate 2 sulfatase Monoclonal antibody

Iduronate 2 sulfatase Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A3F9

Cat no : 66112-1-Ig

Print datasheet

Synonyms

IDS, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase, EC:3.1.6.13, Alpha-L-iduronate sulfate sulfatase

验证数据展示

  • WB analysis of human placenta using 66112-1-Ig

    WB analysis of human placenta using 66112-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66112-1-Ig

    IHC staining of human liver using 66112-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50.

  • IHC staining of human liver using 66112-1-Ig

    IHC staining of human liver using 66112-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50.

  • IF Staining of HepG2 using 66112-1-Ig

    IF Staining of HepG2 using 66112-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Iduronate 2 sulfatase antibody (66112-1-Ig, Clone: 1A3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHuman placenta, rat brain tissue, human brain tissue, mouse brain tissue, pig brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66112-1-Ig targets Iduronate 2 sulfatase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Iduronate 2 sulfatase fusion protein Ag19095 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name iduronate 2-sulfatase
Synonyms IDS, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase, EC:3.1.6.13, Alpha-L-iduronate sulfate sulfatase
Calculated Molecular Weight312aa,35 kDa; 550aa,62 kDa
Observed Molecular Weight 76 kDa, 55 kDa
GenBank Accession NumberBC006170
Gene Symbol IDS
Gene ID (NCBI) 3423
RRIDAB_2881511
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22304
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).

实验方案

Product Specific Protocols
WB protocol for Iduronate 2 sulfatase antibody 66112-1-IgDownload protocol
IHC protocol for Iduronate 2 sulfatase antibody 66112-1-IgDownload protocol
IF protocol for Iduronate 2 sulfatase antibody 66112-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols