验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66112-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66112 targets Iduronate 2 sulfatase in IF/ICC applications and shows reactivity with human, mouse, rat , pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat , pig |
| 免疫原 | Iduronate 2 sulfatase fusion protein Ag19095 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | iduronate 2-sulfatase |
| 别名 | IDS, iduronate 2 sulfatase, Idursulfase, MPS2, SIDS |
| 计算分子量 | 312aa,35 kDa; 550aa,62 kDa |
| 观测分子量 | 76 kDa, 55 kDa |
| GenBank蛋白编号 | BC006170 |
| 基因名称 | IDS |
| Gene ID (NCBI) | 3423 |
| RRID | AB_3084207 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P22304 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Iduronate 2 sulfatase antibody CL488-66112 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |