验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, rat liver tissue, HSC-T6 cells, human kidney tissue, human blood tissue |
| Positive IHC detected in | human ovary tumor tissue, human placenta tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy Ceruloplasmin/CP Ready-To-Use IHC Kit. Ceruloplasmin/CP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
66156-1-Ig targets Ceruloplasmin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Ceruloplasmin fusion protein Ag15535 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ceruloplasmin (ferroxidase) |
| 别名 | Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase |
| 计算分子量 | 1065 aa, 122 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC146663 |
| 基因名称 | Ceruloplasmin |
| Gene ID (NCBI) | 1356 |
| RRID | AB_2881552 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P00450 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Ceruloplasmin antibody 66156-1-Ig | Download protocol |
| IHC protocol for Ceruloplasmin antibody 66156-1-Ig | Download protocol |
| IF protocol for Ceruloplasmin antibody 66156-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest. | ||
FEBS Open Bio Ceruloplasmin overexpression is associated with oncogenic pathways and poorer survival rates in clear-cell renal cell carcinoma. | ||
Clin Immunol Proteomic profiling of aqueous humor-derived exosomes in Vogt-Koyanagi-Harada disease and Behcet's uveitis | ||
Arch Toxicol Arylacetamide deacetylase regulates hepatic iron homeostasis to protect against carbon tetrachloride-induced ferroptosis |
