验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy Ceruloplasmin/CP Ready-To-Use IHC Kit. Ceruloplasmin/CP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66156-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66156 targets Ceruloplasmin in IF/ICC applications and shows reactivity with human, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | Ceruloplasmin fusion protein Ag15535 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ceruloplasmin (ferroxidase) |
| 别名 | Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase |
| 计算分子量 | 1065 aa, 122 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC146663 |
| 基因名称 | Ceruloplasmin |
| Gene ID (NCBI) | 1356 |
| RRID | AB_3672906 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P00450 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Ceruloplasmin antibody CL488-66156 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |