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CoraLite®594-conjugated Ceruloplasmin Monoclonal antibody

Ceruloplasmin Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C8A5

Cat no : CL594-66156

Print datasheet

Synonyms

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

验证数据展示

  • IF Staining of HeLa using CL594-66156

    IF Staining of HeLa using CL594-66156

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Ceruloplasmin antibody (CL594-66156, Clone: 3C8A5 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Ceruloplasmin/CP Ready-To-Use IHC Kit. Ceruloplasmin/CP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66156-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66156 targets Ceruloplasmin in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Ceruloplasmin fusion protein Ag15535 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ceruloplasmin (ferroxidase)
Synonyms Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase
Calculated Molecular Weight 1065 aa, 122 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC146663
Gene Symbol Ceruloplasmin
Gene ID (NCBI) 1356
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00450
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

实验方案

Product Specific Protocols
IF protocol for CL594 Ceruloplasmin antibody CL594-66156Download protocol
Standard Protocols
Click here to view our Standard Protocols