验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human liver tissue, human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | U-937 cells |
Planning an IHC experiment? We recommend our IHCeasy GC/VTDB Ready-To-Use IHC Kit. GC/VTDB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66175-1-Ig targets Vitamin D binding protein in WB, IHC, FC (Intra), CoIP, ChIP, ELISA, IF applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP, ChIP, IF |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | Vitamin D binding protein fusion protein Ag9803 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | group-specific component (vitamin D binding protein) |
| 别名 | GC, VDBP,GC, Gc protein-derived macrophage activating factor, Gc globulin, DBP-maf |
| 计算分子量 | 474 aa, 53 kDa |
| 观测分子量 | 52-58 kDa |
| GenBank蛋白编号 | BC057228 |
| 基因名称 | Vitamin D binding protein |
| Gene ID (NCBI) | 2638 |
| RRID | AB_2881570 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P02774 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Vitamin D binding protein antibody 66175-1-Ig | Download protocol |
| IHC protocol for Vitamin D binding protein antibody 66175-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |