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CoraLite® Plus 488-conjugated Vitamin D binding protein Monoclonal antibody

Vitamin D binding protein Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E4D10

Cat no : CL488-66175

Print datasheet

Synonyms

GC, VDBP,GC, Gc globulin, DBP-maf, DBP/GC

验证数据展示

  • IF Staining of U-937 using CL488-66175

    IF Staining of U-937 using CL488-66175

    Immunofluorescent analysis of (4% PFA) fixed U-937 cells using CoraLite® Plus 488 Vitamin D binding protein antibody (CL488-66175, Clone: 1E4D10 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inU-937 cells
Planning an IHC experiment? We recommend our IHCeasy GC/VTDB Ready-To-Use IHC Kit. GC/VTDB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66175-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66175 targets Vitamin D binding protein in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Vitamin D binding protein fusion protein Ag9803 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name group-specific component (vitamin D binding protein)
Synonyms GC, VDBP,GC, Gc globulin, DBP-maf, DBP/GC
Calculated Molecular Weight 474 aa, 53 kDa
Observed Molecular Weight 52-58 kDa
GenBank Accession NumberBC057228
Gene Symbol Vitamin D binding protein
Gene ID (NCBI) 2638
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02774
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Vitamin D binding protein antibody CL488-66175Download protocol
Standard Protocols
Click here to view our Standard Protocols