验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U-937 cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy GC/VTDB Ready-To-Use IHC Kit. GC/VTDB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66175-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66175 targets Vitamin D binding protein in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | Vitamin D binding protein fusion protein Ag9803 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | group-specific component (vitamin D binding protein) |
| 别名 | GC, VDBP,GC, Gc globulin, DBP-maf, DBP/GC |
| 计算分子量 | 474 aa, 53 kDa |
| 观测分子量 | 52-58 kDa |
| GenBank蛋白编号 | BC057228 |
| 基因名称 | Vitamin D binding protein |
| Gene ID (NCBI) | 2638 |
| RRID | AB_3672907 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P02774 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Vitamin D binding protein antibody CL488-66175 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |