Vitamin D binding protein Monoclonal antibody
Vitamin D binding protein Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
WB, IHC, FC (Intra), ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
1E4D10
验证数据展示
经过测试的应用
Positive WB detected in | human testis tissue |
Positive IHC detected in | human liver tissue, human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | U-937 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66175-1-Ig targets Vitamin D binding protein in WB, IHC, FC (Intra), CoIP, ChIP, ELISA, IF applications and shows reactivity with human samples.
Tested Applications | WB, IHC, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, CoIP, ChIP, IF |
Tested Reactivity | human |
Cited Reactivity | mouse |
Immunogen | Vitamin D binding protein fusion protein Ag9803 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | group-specific component (vitamin D binding protein) |
Synonyms | GC, VDBP,GC, Gc protein-derived macrophage activating factor, Gc globulin, DBP-maf |
Calculated Molecular Weight | 474 aa, 53 kDa |
Observed Molecular Weight | 52-58 kDa |
GenBank Accession Number | BC057228 |
Gene Symbol | Vitamin D binding protein |
Gene ID (NCBI) | 2638 |
RRID | AB_2881570 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P02774 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Vitamin D binding protein antibody 66175-1-Ig | Download protocol |
IHC protocol for Vitamin D binding protein antibody 66175-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |