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Vitamin D binding protein Monoclonal antibody, PBS Only

Vitamin D binding protein Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1E4D10

Cat no : 66175-1-PBS

Print datasheet

Synonyms

GC, VDBP,GC, Gc protein-derived macrophage activating factor, Gc globulin, DBP-maf

验证数据展示

  • WB analysis of human testis using 66175-1-Ig (same clone as 66175-1-PBS)

    WB analysis of human testis using 66175-1-Ig (same clone as 66175-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66175-1-Ig (Vitamin D binding protein antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66175-1-Ig (same clone as 66175-1-PBS)

    IHC staining of human liver using 66175-1-Ig (same clone as 66175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GC/VTDB antibody included
  • IHC staining of human colon cancer using 66175-1-Ig (same clone as 66175-1-PBS)

    IHC staining of human colon cancer using 66175-1-Ig (same clone as 66175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GC/VTDB antibody included
  • IHC staining of human liver cancer using 66175-1-Ig (same clone as 66175-1-PBS)

    IHC staining of human liver cancer using 66175-1-Ig (same clone as 66175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66175-1-Ig (Vitamin D binding protein antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GC/VTDB antibody included
  • FC experiment of U-937 using 66175-1-Ig (same clone as 66175-1-PBS)

    FC experiment of U-937 using 66175-1-Ig (same clone as 66175-1-PBS)

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human Vitamin D binding protein (66175-1-Ig, Clone:1E4D10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66175-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GC/VTDB Ready-To-Use IHC Kit. GC/VTDB primary antibody included.

产品信息

66175-1-PBS targets Vitamin D binding protein in Indirect ELISA, IHC, WB applications and shows reactivity with human samples.

Tested Applications Indirect ELISA, IHC, WB Application Description
Tested Reactivity human
Immunogen Vitamin D binding protein fusion protein Ag9803 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name group-specific component (vitamin D binding protein)
Synonyms GC, VDBP,GC, Gc protein-derived macrophage activating factor, Gc globulin, DBP-maf
Calculated Molecular Weight 474 aa, 53 kDa
Observed Molecular Weight 52-58 kDa
GenBank Accession NumberBC057228
Gene Symbol Vitamin D binding protein
Gene ID (NCBI) 2638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02774
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.