验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells, Jurkat cells, ROS1728 cells, NIH/3T3 cells, 4T1 cells, A431 cells, PC-3 cells, DU145 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 1 publications below |
产品信息
66476-1-Ig targets EZH2 in WB, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | EZH2 fusion protein Ag16789 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | enhancer of zeste homolog 2 (Drosophila) |
| 别名 | EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2, EC:2.1.1.356 |
| 计算分子量 | 751 aa, 86 kDa |
| 观测分子量 | 90-102 kDa |
| GenBank蛋白编号 | BC010858 |
| 基因名称 | EZH2 |
| Gene ID (NCBI) | 2146 |
| RRID | AB_2881842 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15910 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EZH2 antibody 66476-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis Long noncoding RNA MEG3 regulates LATS2 by promoting the ubiquitination of EZH2 and inhibits proliferation and invasion in gallbladder cancer. | ||
Front Cell Dev Biol Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery | ||
Cancer Med LncRNA SPRY4-IT1 facilitates cell proliferation and angiogenesis of glioma via the miR-101-3p/EZH2/VEGFA signaling axis | ||
Int Immunopharmacol Celastrol attenuates diabetic nephropathy by upregulating SIRT1-mediated inhibition of EZH2related wnt/β-catenin signaling | ||
Bioact Mater Engineered mesenchymal stem cell-derived small extracellular vesicles for diabetic retinopathy therapy through HIF-1α/EZH2/PGC-1α pathway | ||
Int Immunopharmacol Characterization of PANoptosis-related genes with immunoregulatory features in osteoarthritis |
