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  • Featured Product
  • KD/KO Validated

EZH2 Monoclonal antibody, PBS Only

EZH2 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F10A12

Cat no : 66476-1-PBS

Print datasheet

Synonyms

EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2, EC:2.1.1.356

验证数据展示

  • WB analysis using 66476-1-Ig (same clone as 66476-1-PBS)

    WB analysis using 66476-1-Ig (same clone as 66476-1-PBS)

    Various cell lines were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66476-1-Ig (same clone as 66476-1-PBS)

    WB analysis of HEK-293 using 66476-1-Ig (same clone as 66476-1-PBS)

    WB result of EZH2 antibody (66476-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EZH2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66476-1-Ig (same clone as 66476-1-PBS)

    FC experiment of HepG2 using 66476-1-Ig (same clone as 66476-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human EZH2 (66476-1-Ig, Clone:1F10A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

  • WB analysis using 66476-1-Ig (same clone as 66476-1-PBS)

    WB analysis using 66476-1-Ig (same clone as 66476-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

产品信息

66476-1-PBS targets EZH2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EZH2 fusion protein Ag16789 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name enhancer of zeste homolog 2 (Drosophila)
Synonyms EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2, EC:2.1.1.356
Calculated Molecular Weight 751 aa, 86 kDa
Observed Molecular Weight 90-102 kDa
GenBank Accession NumberBC010858
Gene Symbol EZH2
Gene ID (NCBI) 2146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15910
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)