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验证数据展示

  • WB analysis using 66476-1-Ig (same clone as 66476-1-PBS)

    WB analysis using 66476-1-Ig (same clone as 66476-1-PBS)

    Various cell lines were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66476-1-Ig (same clone as 66476-1-PBS)

    WB analysis of HEK-293 using 66476-1-Ig (same clone as 66476-1-PBS)

    WB result of EZH2 antibody (66476-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EZH2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66476-1-Ig (same clone as 66476-1-PBS)

    FC experiment of HepG2 using 66476-1-Ig (same clone as 66476-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human EZH2 (66476-1-Ig, Clone:1F10A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

  • WB analysis using 66476-1-Ig (same clone as 66476-1-PBS)

    WB analysis using 66476-1-Ig (same clone as 66476-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EZH2 Monoclonal antibody, PBS Only

EZH2 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 66476-1-PBS

产品说明书

CloneNo. 1F10A12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2, EC:2.1.1.356

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66476-1-PBS targets EZH2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EZH2 fusion protein Ag16789 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 enhancer of zeste homolog 2 (Drosophila)
别名 EZH1, ENX-1, ENX 1, Enhancer of zeste homolog 2, EC:2.1.1.356
计算分子量 751 aa, 86 kDa
观测分子量 90-102 kDa
GenBank蛋白编号BC010858
基因名称 EZH2
Gene ID (NCBI) 2146
RRIDAB_2881842
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15910
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)