验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, human placenta tissue, rat serum tissue, pig plasma tissue |
| Positive IHC detected in | human liver tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HPX Ready-To-Use IHC Kit. HPX primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:40000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
66479-1-Ig targets Hemopexin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, rat, pig |
| 文献引用反应性 | human |
| 免疫原 | Hemopexin fusion protein Ag8533 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | hemopexin |
| 别名 | HPX, Beta-1B-glycoprotein, Beta 1B glycoprotein, 3A9D6 |
| 计算分子量 | 254 aa, 29 kDa |
| 观测分子量 | 65-75 kDa |
| GenBank蛋白编号 | BC005395 |
| 基因名称 | Hemopexin |
| Gene ID (NCBI) | 3263 |
| RRID | AB_2881845 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P02790 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Hemopexin antibody 66479-1-Ig | Download protocol |
| IHC protocol for Hemopexin antibody 66479-1-Ig | Download protocol |
| IF protocol for Hemopexin antibody 66479-1-Ig | Download protocol |
| FC protocol for Hemopexin antibody 66479-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
