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Hemopexin Monoclonal antibody, PBS Only

Hemopexin Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

pig, rat, human

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A9D6

Cat no : 66479-1-PBS

Print datasheet

Synonyms

HPX, Beta-1B-glycoprotein, Beta 1B glycoprotein, 3A9D6

验证数据展示

  • WB analysis of human plasma using 66479-1-Ig (same clone as 66479-1-PBS)

    WB analysis of human plasma using 66479-1-Ig (same clone as 66479-1-PBS)

    0.7 μL human plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

    IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPX antibody included
  • IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

    IHC staining of human liver using 66479-1-Ig (same clone as 66479-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPX antibody included
  • FC experiment of HepG2 using 66479-1-Ig (same clone as 66479-1-PBS)

    FC experiment of HepG2 using 66479-1-Ig (same clone as 66479-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human Hemopexin (66479-1-Ig, Clone:3A9D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66479-1-Ig (same clone as 66479-1-PBS)

    IF Staining of human liver cancer using 66479-1-Ig (same clone as 66479-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Hemopexin antibody (66479-1-Ig, Clone: 3A9D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66479-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HPX Ready-To-Use IHC Kit. HPX primary antibody included.

产品信息

66479-1-PBS targets Hemopexin in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with pig, rat, human samples.

Tested Applications WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity pig, rat, human
Immunogen Hemopexin fusion protein Ag8533 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hemopexin
Synonyms HPX, Beta-1B-glycoprotein, Beta 1B glycoprotein, 3A9D6
Calculated Molecular Weight 254 aa, 29 kDa
Observed Molecular Weight 65-75 kDa
GenBank Accession NumberBC005395
Gene Symbol Hemopexin
Gene ID (NCBI) 3263
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02790
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.