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Hemopexin Monoclonal antibody

Hemopexin Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3A9D6

Cat no : 66479-1-Ig

Print datasheet

Synonyms

HPX, Beta-1B-glycoprotein, Beta 1B glycoprotein, 3A9D6

验证数据展示

  • WB analysis of human plasma using 66479-1-Ig

    WB analysis of human plasma using 66479-1-Ig

    0.7 μL human plasma was subjected to SDS PAGE followed by western blot with 66479-1-Ig (Hemopexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66479-1-Ig

    IHC staining of human liver using 66479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 10x lens).

    Ready-to-use IHC kits available with HPX antibody included
  • IHC staining of human liver using 66479-1-Ig

    IHC staining of human liver using 66479-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66479-1-Ig (Hemopexin antibody) at dilution of 1:500 (under 40x lens).

    Ready-to-use IHC kits available with HPX antibody included
  • FC experiment of HepG2 using 66479-1-Ig

    FC experiment of HepG2 using 66479-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human Hemopexin (66479-1-Ig, Clone:3A9D6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of human liver cancer using 66479-1-Ig

    IF Staining of human liver cancer using 66479-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Hemopexin antibody (66479-1-Ig, Clone: 3A9D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma tissue, human placenta tissue, rat serum tissue, pig plasma tissue
Positive IHC detected inhuman liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HPX Ready-To-Use IHC Kit. HPX primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

66479-1-Ig targets Hemopexin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, rat, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, rat, pig
Cited Reactivityhuman
Immunogen Hemopexin fusion protein Ag8533 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hemopexin
Synonyms HPX, Beta-1B-glycoprotein, Beta 1B glycoprotein, 3A9D6
Calculated Molecular Weight 254 aa, 29 kDa
Observed Molecular Weight 65-75 kDa
GenBank Accession NumberBC005395
Gene Symbol Hemopexin
Gene ID (NCBI) 3263
RRIDAB_2881845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.

实验方案

Product Specific Protocols
WB protocol for Hemopexin antibody 66479-1-IgDownload protocol
IHC protocol for Hemopexin antibody 66479-1-IgDownload protocol
IF protocol for Hemopexin antibody 66479-1-IgDownload protocol
FC protocol for Hemopexin antibody 66479-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
View Article