验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HPX Ready-To-Use IHC Kit. HPX primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66479-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66479 targets Hemopexin in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 | Hemopexin fusion protein Ag8533 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | hemopexin |
| 别名 | HPX, Beta-1B-glycoprotein, Beta 1B glycoprotein, 3A9D6 |
| 计算分子量 | 254 aa, 29 kDa |
| 观测分子量 | 65-75 kDa |
| GenBank蛋白编号 | BC005395 |
| 基因名称 | Hemopexin |
| Gene ID (NCBI) | 3263 |
| RRID | AB_3084250 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P02790 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 Hemopexin antibody CL488-66479 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |