MAdCAM1 Monoclonal antibody
MAdCAM1 Monoclonal Antibody for IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (1)
Applications
IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2A12E8
验证数据展示
经过测试的应用
| Positive IHC detected in | human colon tissue, human tonsillitis tissue, human appendicitis tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Planning an IHC experiment? We recommend our IHCeasy MAdCAM1 Ready-To-Use IHC Kit. MAdCAM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
产品信息
66594-1-Ig targets MAdCAM1 in IHC, IF-P, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IHC, IF-P, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | MAdCAM1 fusion protein Ag16184 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mucosal vascular addressin cell adhesion molecule 1 |
| Synonyms | hMAdCAM 1, MACAM1, MAdCAM 1, MAdCAM1 |
| Calculated Molecular Weight | 406 aa, 43 kDa |
| GenBank Accession Number | BC142629 |
| Gene Symbol | MADCAM1 |
| Gene ID (NCBI) | 8174 |
| RRID | AB_2881954 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13477 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAdCAM1 antibody 66594-1-Ig | Download protocol |
| IF protocol for MAdCAM1 antibody 66594-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Crohns Colitis Mucosal addressin cell adhesion molecule 1 expression reflects mucosal inflammation and subsequent relapse in patients with ulcerative colitis | ||
Int J Cancer MAdCAM-1 targeting strategy can prevent colitic cancer carcinogenesis and progression via suppression of immune cell infiltration and inflammatory signals |
