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NEK2 Monoclonal antibody

NEK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

3D7B4

Cat no : 66632-1-Ig

Print datasheet

Synonyms

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

验证数据展示

  • IF Staining of A431 using 66632-1-Ig

    IF Staining of A431 using 66632-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NEK2 antibody (66632-1-Ig, Clone: 3D7B4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis of human peripheral blood leukocyte using 66632-1-Ig

    WB analysis of human peripheral blood leukocyte using 66632-1-Ig

    Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 66632-1-Ig

    IHC staining of human testis using 66632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK2 antibody included
  • IHC staining of human testis using 66632-1-Ig

    IHC staining of human testis using 66632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK2 antibody included

经过测试的应用

Positive WB detected inHuman peripheral blood leukocyte, THP-1 cells, K-562 cells, HL-60 cells, Jurkat cells, RAW 264.7 cells, rat thymus tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy NEK2 Ready-To-Use IHC Kit. NEK2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66632-1-Ig targets NEK2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, CoIP
Tested Reactivity Human, Mouse , Rat
Cited Reactivityhuman, rat
Immunogen NEK2 fusion protein Ag21476 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NIMA (never in mitosis gene a)-related kinase 2
Synonyms HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC043502
Gene Symbol NEK2
Gene ID (NCBI) 4751
RRIDAB_2881991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP51955
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NEK2 antibody 66632-1-IgDownload protocol
IHC protocol for NEK2 antibody 66632-1-IgDownload protocol
IF protocol for NEK2 antibody 66632-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Toxicol Lett

Oxcarbazepine induces mitotic catastrophe and apoptosis in NRK-52E proximal tubular cells.

Authors - Momoka Ota
View Article
humanWB,IF,CoIP

Cancer Cell Int

NEK2 contributes to radioresistance in esophageal squamous cell carcinoma by inducing protective autophagy via regulating TRIM21

Authors - Dong Guo
  • KD Validated
View Article