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NEK2 Monoclonal antibody, PBS Only

NEK2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D7B4

Cat no : 66632-1-PBS

Print datasheet

Synonyms

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

验证数据展示

  • IF Staining of A431 using 66632-1-Ig (same clone as 66632-1-PBS)

    IF Staining of A431 using 66632-1-Ig (same clone as 66632-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NEK2 antibody (66632-1-Ig, Clone: 3D7B4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

  • WB analysis of human peripheral blood leukocyte using 66632-1-Ig (same clone as 66632-1-PBS)

    WB analysis of human peripheral blood leukocyte using 66632-1-Ig (same clone as 66632-1-PBS)

    Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

    IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK2 antibody included
  • IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

    IHC staining of human testis using 66632-1-Ig (same clone as 66632-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66632-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NEK2 antibody included
Planning an IHC experiment? We recommend our IHCeasy NEK2 Ready-To-Use IHC Kit. NEK2 primary antibody included.

产品信息

66632-1-PBS targets NEK2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse , Rat
Immunogen NEK2 fusion protein Ag21476 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NIMA (never in mitosis gene a)-related kinase 2
Synonyms HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC043502
Gene Symbol NEK2
Gene ID (NCBI) 4751
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP51955
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C