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验证数据展示

  • IF Staining of A431 using 66632-1-Ig

    IF Staining of A431 using 66632-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NEK2 antibody (66632-1-Ig, Clone: 3D7B4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis of human peripheral blood leukocyte using 66632-1-Ig

    WB analysis of human peripheral blood leukocyte using 66632-1-Ig

    Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66632-1-Ig (NEK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 66632-1-Ig

    IHC staining of human testis using 66632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK2 antibody included
  • IHC staining of human testis using 66632-1-Ig

    IHC staining of human testis using 66632-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66632-1-Ig (NEK2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEK2 antibody included

NEK2 Monoclonal antibody

NEK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66632-1-Ig

产品说明书

CloneNo. 3D7B4
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse , Rat and More (2)

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHuman peripheral blood leukocyte, THP-1 cells, K-562 cells, HL-60 cells, Jurkat cells, RAW 264.7 cells, rat thymus tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy NEK2 Ready-To-Use IHC Kit. NEK2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66632-1-Ig targets NEK2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, CoIP
经测试反应性 Human, Mouse , Rat
文献引用反应性human, rat
免疫原 NEK2 fusion protein Ag21476 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NIMA (never in mitosis gene a)-related kinase 2
别名 HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1
计算分子量 52 kDa
观测分子量 48 kDa
GenBank蛋白编号BC043502
基因名称 NEK2
Gene ID (NCBI) 4751
RRIDAB_2881991
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP51955
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NEK2 antibody 66632-1-IgDownload protocol
IHC protocol for NEK2 antibody 66632-1-IgDownload protocol
IF protocol for NEK2 antibody 66632-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Toxicol Lett

Oxcarbazepine induces mitotic catastrophe and apoptosis in NRK-52E proximal tubular cells.

Authors - Momoka Ota
View Article
humanWB,IF,CoIP

Cancer Cell Int

NEK2 contributes to radioresistance in esophageal squamous cell carcinoma by inducing protective autophagy via regulating TRIM21

Authors - Dong Guo
  • KD Validated
View Article